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NEET2026ZoologyBiotechnology - Principles and ProcessesActual

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :

Options

  1. AExtension Annealing Denaturation
  2. BAnnealing Denaturation Extension
  3. CDenaturation Extension Annealing
  4. DDenaturation Annealing Extension

Correct answer

D. Denaturation Annealing Extension

Step-by-step solution

Polymerase Chain Reaction (PCR) involves three main steps in each cycle: Denaturation: The double-stranded DNA is heated to a high temperature (around 94^ C ) to break the hydrogen bonds, resulting in two single strands. Annealing: The temperature is lowered (around 50^ C to 65^ C ) to allow the primers to bind to the complementary sequences on the single-stranded DNA. Extension: The temperature is raised (around 72^ C ) to allow Taq polymerase to synthesize the new DNA strand by adding nucleotides to the primers.

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