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NEET2024ZoologyBiotechnology - Principles and ProcessesActual

Identify the incorrect statement related to gel electrophoresis.

Options

  1. ASeparated DNA fragments can be directly seen under UV radiation
  2. BSeparated DNA can be extracted from gel piece
  3. CFragment of DNA moves toward anode
  4. DSieving effect of agarose gel helps in separation of DNA fragments

Correct answer

A. Separated DNA fragments can be directly seen under UV radiation

Step-by-step solution

Option (1) is the correct answer because separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation. We cannot see pure DNA fragments in visible light and without staining. The DNA fragments separate (resolve) according to their size through sieving effect provided by agarose gel. DNA is a negatively charged molecule, hence it moves towards the positive electrode (anode).

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