Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tet ^R ) gene in the pBR322 cloning vector.

Step-by-step solution

The correct answer is option (1), because the restriction sites for restriction endonucleases Bam HI and Sal I are present within the tet ^R gene in the pBR322 cloning vector. Options (2), (3) and (4) are incorrect because the restriction sites of P st I and P v u I are present within the a m p^R gene in the pBR322 cloning vector.