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Which of the following are correct about EcoRI? A. Cut the DNA with blunt end B. Cut the DNA with sticky end C. Recognise a specific palindromic sequence D. Cut the DNA between the base G and A when encounters the DNA sequence 'GAATTC' E. Exonuclease Choose the correct answer from the options given below:

Options

  1. AB, C, E only
  2. BA, D, E only
  3. CA, C, D only
  4. DB, C, D only

Correct answer

D. B, C, D only

Step-by-step solution

The correct answer is option (4) as EcoRI does not cut the DNA with blunt ends. Instead, it cuts the DNA with sticky / cohesive / staggered ends on each strand. EcoRI is a restriction endonuclease that recognises a specific palindromic sequence and cuts at a specific site within the DNA, known as the restriction site. It is not an exonuclease as exonucleases remove nucleotides from the free ends of the DNA. The recognition sequence for EcoRI is aligned & 5^ - G ^ - A - A - T - T - C -3^ & 3^ - C - T - T - A - A _ -

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