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NEET2024ZoologyBiotechnology - Principles and ProcessesActual

The following diagram showing restriction sites in E . coli cloning vector pBR322. Find the role of ' X and ' Y genes:

Options

  1. AThe gene ' X ' is responsible for controlling the copy number of the linked DNA and ' Y ' for protein involved
  2. BThe gene ' X ' is for protein involved in replication of Plasmid and ' Y ' for resistance to antibiotics.
  3. CGene ' X ' is responsible for recognitions sites and ' Y 's responsible for antibiotic resistance.
  4. DThe gene ' X ' is responsible for resistance to antibiotics and ' Y ' for protein involved in the replication

Correct answer

A. The gene ' X ' is responsible for controlling the copy number of the linked DNA and ' Y ' for protein involved

Step-by-step solution

Correct answer is option (1), because ' X ' in the given diagram is ori while ' Y ' is rop. ' X ' which is ori is responsible for controlling the copy number of the linked DNA and ' Y which is rop codes for protein involved in the replication of plasmid. Options (2), (3) and (4) are incorrect as ' X ' and ' Y ' are not related to these functions.

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