Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?

Step-by-step solution

Option (2) is the answer because, In pBR322, the restriction site for B a m H I is present in tet ^ R gene. Therefore, ligation of a foreign DNA will results in loss of tetracycline resistance and recombinants will be tetracycline sensitive. Option (1) is not the answer because, In pBR322, the restriction site of EcoR I is present outside the region of a m p^R gene and tet ^R gene. Option (3) and (4) are not the answers because, in pBR322, the restriction sites for P s t I and P v u I are present in amp ^ R gene. S