Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEET2023ZoologyBiotechnology - Principles and ProcessesActual

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. A. Insertion of recombinant DNA into the host cell. B. Cutting of DNA at specific location by restriction enzyme. C. Isolation of desired DNA fragment. D. Amplification of gene of interest using PCR. Choose the correct answer from the options given below:

Options

  1. AB, D, A. C
  2. BC, A, B, D
  3. CB, C, D, A
  4. DC, B, D, A

Correct answer

C. B, C, D, A

Step-by-step solution

The process of forming a recombinant DNA molecule involves the following steps: Isolation of gene ot interest or a desired DNA molecule from an organism. Restriction digestion of the desired DNA into fragments to insert into the vector. This is carried out by restriction enzymes. Isolation of these fragments of DNA (desired fragment) The fragments can be separated by gel electrophoresis. Amplification of the gene by PCR thermo-cycler. Insertion of recombinant DNA into the host cell.

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs