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Which one of the following statement is not true regarding gel electrophoresis technique?

Options

  1. AThe separated DNA fragments are stained by using ethidium bromide.
  2. BThe presence of chromogenic substrate gives blue coloured DNA bands on the gel.
  3. CBright orange coloured bands of DNA can be observed in the gel when exposed to UV light.
  4. DThe process of extraction of separated DNA strands from gel is called elution.

Correct answer

B. The presence of chromogenic substrate gives blue coloured DNA bands on the gel.

Step-by-step solution

Isolation of the DNA (or gene of interest) followed by the separation of digested fragment of the DNA is one of the crucial steps of Recombinant DNA Technology. The separation is based on the technique called gel electrophoresis. Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. The separated DNA segments are stained with ethidium bromide for visualisation after exposure to UV light in gel electrophoresis. Brigh

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