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NEET2022ZoologyBiotechnology - Principles and ProcessesActual

Which of the following is not a desirable feature of a cloning vector?

Options

  1. APresence of a marker gene
  2. BPresence of single restriction enzyme site
  3. CPresence of two or more recognition sites
  4. DPresence of origin of replication

Correct answer

C. Presence of two or more recognition sites

Step-by-step solution

The term cloning vectors refer to the DNA molecules that act as transporting vehicle which carries foreign DNA into a host cell for cloning and expression. An ideal cloning vector should have the desired characteristics like small size (less than 10 kb), polylinker sequence, presence of selectable marker genes, and 'Ori' sequence. The 'Ori' sequence, stands for origin of replication, is responsible for the initiation of replication process. In order to link the foreign DNA, the vector needs to have

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