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NEET2021ZoologyBiotechnology - Principles and ProcessesActual

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

Options

  1. ADenaturation, Annealing, Extension
  2. BDenaturation, Extension, Annealing
  3. CExtension, Denaturation, Annealing
  4. DAnnealing, Denaturation, Extension

Correct answer

A. Denaturation, Annealing, Extension

Step-by-step solution

PCR stands for Polymerase Chain Reaction. In this reaction, multiple copies of the gene (or DNA) of interest is synthesised in vitro using two sets of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. Each cycle of Polymerase chain reaction (PCR) has three steps: (i) Denaturation; (ii) Primer annealing; and (iii) Extension of primers. (i) Denaturation, in which double-stranded DNA templates are heated to separate the strands. (ii) Ann

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