NEET2021ZoologyBiotechnology - Principles and ProcessesActual
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
Options
- AMolecular diagnosis
- BGene amplification
- CPurification of isolated protein
- DDetection of gene mutation
Correct answer
C. Purification of isolated protein
Step-by-step solution
PCR stands for Polymerase Chain Reaction. In this reaction, multiple copies of the gene (or DNA) of interest is synthesised in vitro using two sets of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. Application of PCR are molecular diagnosis, gene amplification, and detection of gene mutation. PCR is now routinely used to detect HIV in suspected AIDS patients. It is being used to detect mutations in genes in suspected cancer patient