During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

Step-by-step solution

Denaturation - 94 - 95 ° C , annealing- 50 - 60 ° C , extension- 72 ° C . Since maximum temperature is required during denaturation, it will be affected the most. The initial denaturation step is carried out at the beginning of PCR to separate the double-stranded template DNA into single strands so that the primers can bind to the target region and initiate extension .