NEET2020ZoologyBiotechnology - Principles and ProcessesActual
Select the correct statement from the following:
Options
- AGel electrophoresis is used for amplification of a DNA segment.
- BThe polymerase enzyme joins the gene of interest and the vector DNA .
- CRestriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes
- DPCR is used for isolation and separation of gene of interest.
Correct answer
C. Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes
Step-by-step solution
Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. Restriction endonuclease identifies the specific restriction sites or palindromic sequences and cut the DNA under optimum conditions. Gel electrophoresis is used for separation of DNA fragments based on size. PCR is used for amplification of a DNA segment. The DNA ligase enzyme joins the gene of interest and the vector DNA .