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NEET2020ZoologyBiotechnology - Principles and ProcessesActual

Match the organism with its use in biotechnology. (a) Bacillus thuringiensis (i) Cloning vector (b) Thermus aquaticus (ii) Construction of first rDNA molecule (c) Agrobacterium tumefaciens (iii) DNA polymerase (d) Salmonella typhimurium (iv) Cry proteins

Options

  1. A(a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
  2. B(a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
  3. C(a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
  4. D(a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)

Correct answer

A. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)

Step-by-step solution

The construction of the first recombinant DNA emerged from the possibility of linking a gene–encoding antibiotic resistance with a native plasmid (autonomously replicating circular extrachromosomal DNA) of Salmonella typhimurium . Stanley Cohen and Herbert Boyer accomplished this in 1972 by isolating the antibiotic resistance gene by cutting out a piece of DNA from a plasmid that was responsible for conferring antibiotic resistance. The tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens has been modifie

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