Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEET2020ZoologyBiotechnology - Principles and ProcessesActual

Choose the correct pair from the following.

Options

  1. APolymerases - Break the DNA into fragments
  2. BNucleases - Separate the two strands of DNA
  3. CExonucleases - Make cuts at specific positions within DNA
  4. DLigases - Join the two DNA molecules

Correct answer

D. Ligases - Join the two DNA molecules

Step-by-step solution

Ligases: These enzymes catalyze the covalent bonding of two substrates to form a large molecule. They catalyze the joining of C–O, C–S, S–N, P–O, etc., bonds by using the energy of ATP. They are also called molecular glue or stickers. Example: DNA ligase. Two DNA molecules are joined together by a phosphodiester bond in the presence of DNA ligases. The enzymes cannot be utilised for the blunt ends of the fragments of DNA. The bond nature is covalent because of the involvement of electron exc

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. 2026Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting 2026Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the 2026Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment 2026Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu 2026In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? 2025Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f 2025Which of the following cannot be used for direct gene transfer? 2019 Full Biotechnology - Principles and Processes list All NEET PYQs