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NEET2020ZoologyBiotechnology - Principles and ProcessesActual

Identify the wrong statement with regard to restriction enzymes.

Options

  1. AThey cut the strand of DNA at palindromic sites.
  2. BThey are useful in genetic engineering.
  3. CSticky ends can be joined by using DNA ligases.
  4. DEach restriction enzyme functions by inspecting the length of a DNA sequence.

Correct answer

C. Sticky ends can be joined by using DNA ligases.

Step-by-step solution

Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single–stranded portions at the ends. There are overhanging stretches called sticky ends or cohesive ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.

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