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NEET2019ZoologyBiotechnology - Principles and ProcessesActual

DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:

Options

  1. AIsopropanol
  2. BChilled ethanol
  3. CMethanol at room temperature
  4. DChilled chloroform

Correct answer

B. Chilled ethanol

Step-by-step solution

DNA is highly polarised due to phosphate backbone. This makes it highly soluble in water. Generally, 0.3 M Sodium Acetate is used which neutralizes the negative charge on the PO3- groups on the nucleic acids, making the molecule far less hydrophilic, and therefore much less soluble in water. Then, three volumes of (at least) 95% ethanol is added that removes the hydration shell of H2O molecules around the phosphate.

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