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NEET2018ZoologyBiotechnology - Principles and ProcessesActual

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

Options

  1. Aλ phage
  2. BTi plasmid
  3. CRetrovirus
  4. DpBR 322

Correct answer

C. Retrovirus

Step-by-step solution

Usually, a retrovirus is used as a vector for introducing a DNA fragment in human cells. They have used as a vector in gene therapy to introduce the desired gene so as to replace the functioning of a defective gene Ex: Severe Combined Immune Deficiency (SCID) is a disease caused due to a defect in the gene for the enzyme adenosine deaminase. In gene therapy against it, lymphocytes are extracted from the bone marrow of the patient. These are grown in a culture outside the body. A functional ADA rDNA, using a retrovi

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