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NEET2018ZoologyBiotechnology - Principles and ProcessesActual

The correct order of steps in Polymerase Chain Reaction (PCR) is

Options

  1. ADenaturation, Extension, Annealing
  2. BAnnealing, Extension, Denaturation
  3. CExtension, Denaturation, Annealing
  4. DDenaturation, Annealing, Extension

Correct answer

D. Denaturation, Annealing, Extension

Step-by-step solution

The Polymerase Chain Reaction (PCR) involves three basic steps; denaturation, annealing and extension. In the denaturation step, DNA is heated at high temperature (94°C to 96°C) to separate the two strands. In the next step (annealing) the two oligonucleotide primers anneal to each single-stranded template DNA. This step is carried out at a lower temperature (40°C to 60°C). The final step is an extension, wherein Taq DNA polymerase synthesizes the DNA region between the primers, using dNTPs (deoxynu

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