NEET2017ZoologyBiotechnology - Principles and ProcessesActual
The DNA fragments separated on an agarose gel can be visualised after staining with:
Options
- ABromophenol blue
- BAcetocarmine
- CAniline blue
- DEthidium bromide
Correct answer
D. Ethidium bromide
Step-by-step solution
The DNA fragments separated on an agarose gel can be visualised after staining with ethidium bromide. It is an intercalating agent and a fluorescent agent. The stained DNA fragments are seen as bright orange-coloured bands under UV light. Intercalation is the insertion of molecules between the planar bases of DNA. This process is used as a method for analysing DNA.