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NEET2015ZoologyBiotechnology - Principles and ProcessesActual

The introduction of T-DNA into plants involves :

Options

  1. AAltering the pH of the soil, then heat-shocking the plants
  2. BExposing the plants to cold for a brief period
  3. CAllowing the plant roots to stand in water
  4. DInfection of the plant by Agrobacterium tumefaciens

Correct answer

D. Infection of the plant by Agrobacterium tumefaciens

Step-by-step solution

Ti plasmid of Agrobacterium tumefaciens: Agrobacterium tumefaciens, a pathogen of several dicot plants is able to deliver a piece of DNA known as ‘T-DNA’ to transform normal plant cells into a tumor and direct these tumor cells to produce the chemicals required by the pathogen. This bacterium invades plants at the site of wound, transforming them and nearby cells to form a tumor called crown gall. When the bacterium contacts a damaged plant cell, it delivers a T-DNA (transferred DNA) fragment from plasm

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