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NEET2014ZoologyBiotechnology - Principles and ProcessesActual

Commonly used vectors for human genome sequencing are:

Options

  1. AT - DNA
  2. BBAC and YAC
  3. CExpression Vectors
  4. DT/A Cloning Vectors

Correct answer

B. BAC and YAC

Step-by-step solution

A bacterial artificial chromosome (BAC) are the plasmids usually applied for the transformation and cloning in bacterial cells, generally E. coli. The usual BAC insert size is around 150-350 Kbp. A similar cloning vector named as PAC has also been developed from the bacterial P1-plasmid. BACs are mostly applied for sequencing purposes in genome projects, as they can be inserted with long size of DNA fragments. Yeast artificial chromosomes (YACs) are genetically engineered chromosomes modified from the DNA of the ye

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