NEET2013ZoologyBiotechnology - Principles and ProcessesActual
Which of the following is a cloning vector?
Options
- ADNA of Salmonella typhimurium
- BTi plasmid
- CAmp ^r and Tet ^r loci
- DOri minus pBR322
Correct answer
B. Ti plasmid
Step-by-step solution
The DNA used as a carrier for transferring a fragment of foreign DNA into a suitable host is called vehicle DNA or cloning vector or gene carrier. The Ti plasmid (tumour inducing plasmid) is present in Agrobacterium tumefaciens, a Gram negative soil bacterium that infects a wide range of plants and causes tumorous growth specially at the root/stem junction (crown gall). The Ti plasmid comprises the gene responsible for the tumorous growth, gets incorporated into the genome of infected plant cells. This property is