NEET2008ZoologyBiotechnology - Principles and ProcessesActual
Assertion : Hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesized by myeloma cells. Reason : This medium allows selection of hybridoma cells.
Options
- AIf both assertion and reason are true and reason is the correct explanation of assertion
- BIf both assertion and reason are true but reason is not the correct explanation of assertion
- CIf assertion is true but reason is false
- DIf both assertion and reason are false.
Correct answer
A. If both assertion and reason are true and reason is the correct explanation of assertion
Step-by-step solution
The hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesized by myeloma cells so that myleoma cells could be removed or hybridoma cells could be purified. The unfused myleoma cells die while hybridoma cells survive. The hybridoma cells are allowed to multiply separately.