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NEET2012ZoologyBiotechnology - Principles and ProcessesActual

Gel electrophoresis is used for

Options

  1. Acutting of DNA into fragments
  2. Bseparation of DNA fragments according to their size
  3. Cconstruction of recombinant DNA by joining with cloning vectors
  4. Disolation of DNA molecule

Correct answer

B. separation of DNA fragments according to their size

Step-by-step solution

When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different sizes can be separated through gel electrophoresis. Gel electrophoresis involves movement of fragments or molecules on the basis if molecule size and shape under the influence of electric field. Endonucleases are enzymes that produce internal cuts called cleavage in DNA molecules. It cleaves DNA only within or near recognition sites, which have specifi

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