NEET2007ZoologyBiotechnology - Principles and ProcessesActual
Which one of the following hydrolysis internal phosphodiester bonds in a polynucleotide chain?
Options
- ALipase
- BExonuclease
- CEndonuclease
- DProtease
Correct answer
C. Endonuclease
Step-by-step solution
DNA ase (or deoxyribonuclease) or simply nuclease is an enzyme which breaks down DNA by hydrolysis of the phosphodiester bonds of its sugar-phosphate back-bone. Depending on the position of hydrolysing phosphodiester bonds, nucleases are of two types: (i) Endonucleases (ii)Exonucleases. Endonucleases hydrolyse internal phosphodiester bonds in a polynucleotide chain (i.e., DNA). While exonucleases hydrolyse terminal phosphodiester bonds in a polynucleotide chain (i.e., DNA).