AIIMS 201827 May
- Exam
- AIIMS
- Year
- 2018
- Date
- 27 May
- Subject
- Zoology
- Chapter
- Biotechnology - Principles and Processes
AIIMS 2018 · 27 May · Zoology · Biotechnology - Principles and Processes
Which of the following statements is not correct regarding EcoRI...
Which of the following statements is not correct regarding EcoRI restriction endonuclease enzyme?
Options
- AIt is isolated from Escherichia coli RY13.
- BIts recognition sequence is -GAATTC- 3 ' 3'-CTTAAG-5'.
- CIt produces complementary blunt ends.
- DNone of these
Correct answer
C. It produces complementary blunt ends.
Step-by-step solution
The restriction endonuclease enzyme EcoRI was isolated from bacterium Escherichia coli RY13. It recognises the base sequence GAATTC in DNA duplex and cuts its strands between and as shown below:
It results in complementary sticky ends.
It results in complementary sticky ends.