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AIIMS 2015
Exam
AIIMS
Year
2015
Subject
Zoology
Chapter
Biotechnology - Principles and Processes

AIIMS 2015 · Zoology · Biotechnology - Principles and Processes

Select the correct order of processing of PCR.

Select the correct order of processing of PCR.

Options

  1. AExtension, primer annealing, denaturation
  2. BDenaturation, primer annealing, extension
  3. CDenaturation, extension, primer annealing
  4. DPrimer annealing, denaturation, extension

Correct answer

B. Denaturation, primer annealing, extension

Step-by-step solution

Polymerase chain reaction (PCR) is a technique to amplify a selected DNA sequence by a million fold or more. In this procedure, the DNA containing the sequence to be amplified is denatured by heating. This denatured DNA is then annealed to the synthetic oligonucleotide primers. Then Taq polymerase is used to replicate the DNA segment between the sites complementary to oligonucleotide primer and extend it.
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