AIIMS 2016
- Exam
- AIIMS
- Year
- 2016
- Subject
- Zoology
- Chapter
- Biotechnology - Principles and Processes
AIIMS 2016 · Zoology · Biotechnology - Principles and Processes
Some of the steps of DNA fingerprinting are given below. Identify...
Some of the steps of DNA fingerprinting are given below. Identify their correct sequence from the options given.
A. Electrophoresis of DNA fragments
B. Hybridisation with DNA probe
C. Digestion of DNA by restriction endonucleases
D. Autoradiography
E. Blotting ofDNA fragmentstonitrocellulose membrane
Options
- A
- B
- C
- D
Correct answer
B.
Step-by-step solution
The technique of DNA fingerprinting involves following steps:
(i) Isolation of DNA from sample cell.
(ii) Amplification of DNA using polymerase chain reaction (PCR), (if DNA is less in amount).
(iii) Digestion of this DNA by restriction endonucleases (C).
(iv) These DNA fragments are electrophoresed in agarose gel. These fragments can be visualised using EtBr-UV system (A).
(v) Separated DNA fragments are transferred to nitrocellulose membrane using Southern Blotting (E).
(vi) Probing for VNTRs is done using labelled DNA probes (B).
(vii)The hybridised fragment can be detected by autoradiography (D).