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AIIMS 2009
Exam
AIIMS
Year
2009
Subject
Zoology
Chapter
Biotechnology - Principles and Processes

AIIMS 2009 · Zoology · Biotechnology - Principles and Processes

Assertion : Restriction enzymes cut the strand of DNA to produce...

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.

Options

  1. AIf both assertion and reason are true and reason is the correct explanation of assertion
  2. BIf both assertion and reason are true but reason is not the correct explanation of assertion
  3. CIf assertion is true but reason is false
  4. DIf both assertion and reason are false.

Correct answer

C. If assertion is true but reason is false

Step-by-step solution

Restriction enzyme, a type of endonuclease, functions by "inspecting" the length of a DNA sequence. Once it finds a recognition sequence, it binds and cut each of the two strands of the double helix at specific point leaving single stranded portions at the ends. This results in overhanging stretches called sticky ends. These in the same individual. These are called bisexual or monoecious animals or hermaphrodites. In these animals, testes and ovaries do not mature simultaneously. For example in earthworm, testes mature earlier, while in others, e.g., sea-squirt, ovaries mature earlier. These conditions are respectively called protandry and protogyny. They ensure cross fertilization. Cross fertilization is a process in which gametes derived from different individuals are united to form zygote. It involves the mixing of two different genetic materials. This is better than self-fertilization as it introduces variation by combining traits of two individuals.
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