AIIMS 2004
- Exam
- AIIMS
- Year
- 2004
- Subject
- Zoology
- Chapter
- Biotechnology - Principles and Processes
AIIMS 2004 · Zoology · Biotechnology - Principles and Processes
What is the firststep in the Southern Blottechnique?
What is the firststep in the Southern Blottechnique?
Options
- ADenaturation of DNA on the gel for hybridization with specific probe
- BProduction of a group of genetically identical cells
- CDigestion of DNA by restriction enzyme
- DIsolation of DNA from a nucleated cell such as the one from the scene of crime
Correct answer
D. Isolation of DNA from a nucleated cell such as the one from the scene of crime
Step-by-step solution
Southern blotting was named after Edward M. Southern who developed this procedure in Edinburgh University in the 1970's. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture. The amount of DNA needed for this technique is dependent on the size and specific activity of the probe. Before, starting the procedure, the desired DNA should be isolated from a specific nucleated cell.