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IAT IISER2026BiologyBiotechnology - Principles and Processes

The pBR322 cloning vector has genes coding for tetracycline and ampicillin resistance. A foreign DNA to be cloned is inserted into the tetracycline resistance gene and the recombinant plasmid is then transformed into E. coli cells. Which one of the following choices is the most likely outcome of this cloning reaction?

Options

  1. AThe cells with the recombinant plasmid can grow in the presence of ampicillin but not tetracycline
  2. BThe cells with the recombinant plasmid can grow in the presence of both ampicillin and tetracycline
  3. CThe cells with the non-recombinant plasmid can grow in the presence of ampicillin but not tetracycline
  4. DThe cells with the non-recombinant plasmid can grow in the presence of tetracycline but not ampicillin

Correct answer

A. The cells with the recombinant plasmid can grow in the presence of ampicillin but not tetracycline

Step-by-step solution

The pBR322 vector contains two antibiotic resistance genes: ampicillin resistance ( amp^ R ) and tetracycline resistance ( tet^ R ). When a foreign DNA is inserted into the tetracycline resistance gene, the gene is disrupted. This phenomenon is known as insertional inactivation. As a result, the recombinant plasmid loses its ability to confer resistance to tetracycline, while the ampicillin resistance gene remains intact and functional. Therefore, E. coli cells transformed with the recombinant plasmid will be able

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