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KCET2026BiologyBiotechnology - Principles and Processes

Given below are two statements. Statement-I: Restriction enzyme BamHI has its recognition site in R^ tet region of pBR322. Statement-II: E.coli having pBR322 with a desired DNA if inserted at BamHI site can grow in medium containing tetracycline. In the light of the above statements, choose the correct answer from the options given below.

Options

  1. ABoth Statement I and Statement II are true
  2. BBoth Statement I and Statement II are false
  3. CStatement I is true, but Statement II is false
  4. DStatement I is false, but Statement II is true

Correct answer

C. Statement I is true, but Statement II is false

Step-by-step solution

In the plasmid pBR322, the tetracycline resistance gene ( tet^ R ) contains recognition sites for the restriction enzymes BamHI and SalI. Therefore, Statement-I is true. When a desired DNA fragment is inserted at the BamHI site, the tetracycline resistance gene gets disrupted due to insertional inactivation. As a result, the recombinant plasmid loses its resistance to tetracycline. Thus, E. coli host cells containing this recombinant pBR322 will not be able to grow in a medium containing tetracycline. Therefore, St

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