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KCET2026BiologyBiotechnology - Principles and Processes

Match List I with List II List I List II a) Visualization of DNA in gel electrophoresis i) Chitinase b) Precipitation of DNA ii) Lysozyme c) Breaking of cell wall of bacteria iii) Chilled ethanol d) Breaking of cell wall of fungus iv) Ethidium bromide Codes:

Options

  1. Aa - iv, b - iii, c - ii, d – i
  2. Ba - iii, b - iv, c - i, d – ii
  3. Ca - iv, b - i, c - ii, d – iii
  4. Da - iii, b - ii, c - iv, d – i

Correct answer

A. a - iv, b - iii, c - ii, d – i

Step-by-step solution

Visualization of DNA in gel electrophoresis requires staining with ethidium bromide followed by exposure to UV radiation. Precipitation of purified DNA is achieved by adding chilled ethanol. The bacterial cell wall is broken down by the enzyme lysozyme to release DNA and other macromolecules. The fungal cell wall, which is made of chitin, is broken down by the enzyme chitinase. Therefore, the correct matching is a - iv, b - iii, c - ii, d - i. Answer: a - iv, b - iii, c - ii, d – i

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