In the plasmid vector map T e t^R denotes tetracycline resistance gene and A m p^R denotes ampicillin resistance gene. A SalI restriction enzyme-digested DNA fragment containing the gene of interest (insert) is ligated to the SalI-digested plasmid vector and then transformed into strain of E . coli that is sensitive to both antibiotics (test reaction). The desired transformants (containing the recombinant plasmid) we

Options

1. ALigation of BamHI-digested plasmid vector with the SalI-digested insert and transformed into E. coli followed
2. BLigation of SalI-digested plasmid vector alone, and transformed into E. coli followed by plating on ampicillin
3. CUnligated SalI-digested plasmid vector alone transformed into E. coli followed by plating on ampicillin contai
4. DUnligated SalI-digested plasmid vector alone transformed into E. coli followed by plating on tetracycline cont