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IAT IISER2021BiologyBiotechnology - Principles and Processes

The map of a 4000 base pair (bp) plasmid DNA marking the locations of different restriction enzyme cut sites is shown in the figure below. The numbers in brackets indicate the base pair positions where the enzymes cut. This plasmid is completely digested first with the combination of restriction enzymes PstI and HindIII, and then with EcoRI and SalI. The final digested plasmid is analyzed by agarose gel electrophores

Options

  1. A220,540,900 and 2340
  2. B220,540,1200 and 2040
  3. C540,760,1200 and 1500
  4. D320,540,800 and 2340

Correct answer

A. 220,540,900 and 2340

Step-by-step solution

No solution available.

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