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IAT IISER2023BiologyBiotechnology - Principles and Processes

A 1000 base-pair DNA fragment was cloned between Hind III and EcoR I sites of the plasmid vector (pIAT) of size 3500 base-pair. The cloned fragment had a Not I site as shown in the figure. In order to confirm the presence of the insert, the recombinant plasmid was digested completely with (a) Not I and EcoR I, and (b) Not I and Hind III. In lane 1 the products of the digestion by Not I and EcoR I was loaded. In lane

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