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IAT IISER2018BiologyBiotechnology - Principles and Processes

A gene is inserted into the PvuII site of the cloning vector (given below) and transformed into E. coli cells. Which one of the following statements is then true?

Options

  1. ARecombinants can be selected by plating on ampicillin containing medium.
  2. BRecombinants can be selected by plating on tetracycline containing medium.
  3. CRecombinants cannot be selected by plating either on ampicillin or tetracycline containing medium.
  4. DRecombinants can be selected by plating simultaneously on ampicillin and tetracycline containing medium.

Correct answer

C. Recombinants cannot be selected by plating either on ampicillin or tetracycline containing medium.

Step-by-step solution

No solution available.

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