For a double strand DNA, one strand is given below: The amount of energy required to split the double strand DNA into two single strands is _______ kcal mol ⁻¹ . [Given: Average energy per H -bond for A-T base pair =1.0 kcal mol ⁻¹, G - C b

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For a double strand DNA, one strand is given below: The amount of energy required to split the double strand DNA into two single strands is _______ kcal mol ⁻¹ . [Given: Average energy per H -bond for A-T base pair =1.0 kcal mol ⁻¹, G - C base pair =1.5 kcal mol ⁻¹ , and A-U base pair =1.25 kcal mol ⁻¹ . Ignore electrostatic repulsion between the phosphate groups.]

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A = T 2 H -bond G C 3 H -bond Number of A = T pair =7 Number of G C pair =6 Number of H-bond involve in A = T =7 2=14 Number of H -bond involve in G C =6 3=18 Energy required for A = T =14 1=14 Energy required for G C =18 1.5=27 Total energy required 14+27=41

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