Which one of the following tests used for the identification of functional groups in organic compounds does not use copper reagent?

Step-by-step solution

Seliwanoff's Test : This test is used to detect the presence of Ketohexose. Reagent : Resorcinol + conc. HCl . Barfoed's Test : It is used to detect Monosaccharide by reduction of Cu ( II ) into Copper (I). Fehling Solution: It is used to detect aldehyde by reduction Cu ( II ) into Cu ( I ) . Biuret Test : Use to detect presence of peptide bond in protein by reduction of Cu ( II ) into Cu ( I ) .