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JIPMER 2007
Exam
JIPMER
Year
2007
Subject
Zoology
Chapter
Biotechnology - Principles and Processes

JIPMER 2007 · Zoology · Biotechnology - Principles and Processes

Which one of the following hydrolysis internal phosphodiester bonds...

Which one of the following hydrolysis internal phosphodiester bonds in a polynucleotide chain?

Options

  1. ALipase
  2. BExonuclease
  3. CEndonuclease
  4. DProtease

Correct answer

C. Endonuclease

Step-by-step solution

DNA ase (or deoxyribonuclease) or simply nuclease is an enzyme which breaks down DNA by hydrolysis of the phosphodiester bonds of its sugar-phosphate back-bone. Depending on the position of hydrolysing phosphodiester bonds, nucleases are of two types: (i) Endonucleases (ii)Exonucleases. Endonucleases hydrolyse internal phosphodiester bonds in a polynucleotide chain (i.e., DNA). While exonucleases hydrolyse terminal phosphodiester bonds in a polynucleotide chain (i.e., DNA).
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