Following are the key steps involved in the overall process of...

The steps in recombinant DNA technology are carried out in a specific sequence:

First, the DNA is isolated and cut at specific locations using restriction endonucleases (Step B).

The gene of interest is then amplified using Polymerase Chain Reaction or PCR (Step D).

The amplified gene is ligated with a vector and the recombinant DNA is inserted into a suitable host cell (Step A).

The host cells are cultured at a large scale to express and obtain the foreign gene product (Step E).

Finally, the product is subjected to downstream processing, which includes separation and purification, before it is ready for marketing (Step C).

The correct sequence is $\mathrm{B} \rightarrow \mathrm{D} \rightarrow \mathrm{A} \rightarrow \mathrm{E} \rightarrow \mathrm{C}$.

Answer: $\mathrm{B} \rightarrow \mathrm{D} \rightarrow \mathrm{A} \rightarrow \mathrm{E} \rightarrow \mathrm{C}$