NEET2008ZoologyBiotechnology - Principles and ProcessesActual
Gel electrophoresis is used for
Options
- Acutting of DNA into fragments
- Bseparation of DNA fragments according to their size
- Cconstruction of recombinant DNA by joining with cloning vectors
- Disolation of DNA molecule
Correct answer
B. separation of DNA fragments according to their size
Step-by-step solution
When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different sizes can be separated through gel electrophoresis. Gel electrophoresis involves movement of fragments or molecules from a well created on one edge of the gel. Endonucleases are enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves DNA only within or near those sites, which have specific base sequences, s