NEST2020BiologyBiotechnology - Principles and Processes
A polymerase chain reaction (PCR) for amplification of DNA fragments involves multiple cycles of thermal denaturation, annealing and extension steps in each cycle. Because of the heat denaturation step, a thermostable polymerase is required for performing PCR. An experimenter had all the resources except the thermostable DNA polymerase to perform a PCR. The experimenter can use ______ to substitute for thermostable D
Options
- AEscherichia coli DNA polymerase added before denaturation step of every PCR cycle
- BEscherichia coli DNA polymerase, added after extension step of every PCR cycle
- Cexcess amount of Escherichia coli DNA polymerase added once at the beginning of the reaction
- DEscherichia coli DNA polymerase, added after annealing step of every PCR cycle
Correct answer
D. Escherichia coli DNA polymerase, added after annealing step of every PCR cycle
Step-by-step solution
No solution available.