Restriction enzymes recognize certain sequences within the DNA and cleave them. If a DNA fragment is cleaved with BamHI restriction enzyme, it generates sticky ends. If the same DNA fragment is cleaved with Sau3A restriction enzyme, it generates sticky ends. The cleaved fragments can be joined using DNA ligase. The recognition and cleavage site (red arrows) for BamHI and Sau3A are given below. N represents any of the

Options

1. AIf a Sau3A cleaved end is ligated to a BamHI cleaved end, the ligated fragment can be further digested using S
2. BIf a BamHI cleaved end is ligated to a Sau3A cleaved end, the ligated fragment can be further digested using B
3. CIf both the recognition sequences are reverse complemented then it cannot be cleaved using either BamHI or Sau
4. DIf a BamHI cleaved end is ligated to a Sau3A cleaved end and reverse complemented then the ligated fragment ca