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NEST2024BiologyBiotechnology - Principles and Processes

In the vector map, GFP denotes the green fluorescent protein, T e t^R is a tetracycline resistance gene and A m p^R is an ampicillin resistance gene. A DNA fragment containing the gene of interest was generated using SalI digestion. This fragment was ligated with the SalI digested vector. Assume complete digestion by SalI. E. coli was transformed with the ligation product and transformants were plated on media. The r

Options

  1. Aampicillin and are GFP positive
  2. Btetracycline and are GFP negative
  3. Campicillin and are GFP negative
  4. Dtetracycline and are GFP positive

Correct answer

B. tetracycline and are GFP negative

Step-by-step solution

No solution available.

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