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NEST2019BiologyBiotechnology - Principles and Processes

A purified linear double-stranded DNA fragment was independently subjected to five treatments using different restriction enzymes (RE) and the unique fragment sizes of DNA thus obtained were measured using conventional agarose gel electrophoresis. The results for such treatments are as depicted in the table below. Which of the following option(s) is/are correct?

Options

  1. ARE site for NdeI lies between those for HindIII and EcoRI.
  2. BRE sites for EcoRI and NdeI are 3 kb apart.
  3. CRE site for HindIII lies between those for EcoRI and NdeI.
  4. DA double digestion with HindIII and N d e I would give rise to 2,3 and 4 kb fragments.

Correct answer

D. A double digestion with HindIII and N d e I would give rise to 2,3 and 4 kb fragments.

Step-by-step solution

No solution available.

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