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In a cloning experiment using E. coli, a DNA fragment was inserted in an EcoRI restriction site of a plasmid vector that contained the kan ^ R and spec ^ R genes for resistance to the antibiotics kanamycin and spectinomycin, respectively. It was observed that all the positive clones (containing the DNA fragment of interest) grew on medium with kanamycin but not on media containing spectinomycin. Colonies, which grew

Options

  1. AThe spec ^R gene contains a mutation which generates a stop codon within the gene.
  2. BThe EcoRI site was located within the spec ^R gene.
  3. CThe cloned DNA fragment was lethal to the cell.
  4. DThe cloned fragment of interest produces a protein which binds and inactivates the protein produced by the spe

Correct answer

D. The cloned fragment of interest produces a protein which binds and inactivates the protein produced by the spe

Step-by-step solution

No solution available.

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