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NEST2022BiologyBiotechnology - Principles and Processes

An experimenter ligated cDNA of p 53 gene into the multiple cloning site (MCS) of a vector whose map is shown below. The ligated vector was transformed into E. coli cells, and transformants were initially selected on an agar plate containing antibiotic P . The bacterial colonies obtained on this plate were further propagated sequentially on antibiotic Q and then antibiotic R by replica plating. The correct sequence(s

Options

  1. AAmpicillin Chloramphenicol Tetracycline.
  2. BChloramphenicol Tetracycline Ampicillin.
  3. CAmpicillin Tetracycline Chloramphenicol.
  4. DTetracycline Chloramphenicol Ampicillin.

Correct answer

D. Tetracycline Chloramphenicol Ampicillin.

Step-by-step solution

No solution available.

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