Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEST2021BiologyBiotechnology - Principles and Processes

In four separate experiments (Table), a vector DNA pBxyz ( 6000 bp ) and a DNA-X (1500 bp) fragment was digested with the indicated restriction enzymes. Both, the digested vector and DNA-X were purified and ligated. A m p^r represents the ampicillin resistant gene and Ori is the origin of replication. The resultant recombinant vector was transformed into Escherichia coli cells and incubated in a liquid nutrient mediu

Options

  1. AIn experiment 4, the transformed Escherichia coli will be ampicillin sensitive, and the recombinant vector can
  2. BIn experiment 2, the transformed Escherichia coli will be ampicillin resistant, and the recombinant vector can
  3. CIn experiment 1, the transformed Escherichia coli will be ampicillin resistant, and the recombinant vector can
  4. DIn experiment 3, the transformed Escherichia coli will be ampicillin sensitive, and the recombinant vector can

Correct answer

B. In experiment 2, the transformed Escherichia coli will be ampicillin resistant, and the recombinant vector can

Step-by-step solution

No solution available.

Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

The pBR322 cloning vector has genes coding for tetracycline and ampicillin resistance. A foreign DNA to be cloned is inserted into the tetracycline resistance gene and the recombin 2026Technique to alter the chemistry of genetic materials, DNA and RNA, to introduce these into host organism and change the phenotype of the host is 2026Given below are two statements. Statement-I: Restriction enzyme BamHI has its recognition site in R^ tet region of pBR322. Statement-II: E.coli having pBR322 with a desired DNA if 2026Match List I with List II List I List II a) Visualization of DNA in gel electrophoresis i) Chitinase b) Precipitation of DNA ii) Lysozyme c) Breaking of cell wall of bacteria iii) 2026A researcher wants to insert a linear DNA fragment into a plasmid DNA. The linear DNA fragment has two MfeI restriction sites at its ends. The plasmid DNA has only one EcoRI restri 2026The given picture was obtained from an agarose gel electrophoresis of a plasmid after digestion with restriction enzymes either X , Y or both X and Y. Which one of the following di 2025Polymerase chain reaction (PCR) is used to amplify a gene of interest (GOI). If, after 30 cycles of PCR, 1 billion copies of GOI are produced, approximately how many copies of GOI 2024Which of the following plasmid vectors can be used for cloning of a gene, with restriction enzymes BamHI and EcoRI, and ampicillin-containing nutrient agar for selection? [Ori and 2024 Full Biotechnology - Principles and Processes list All NEST PYQs